In this study, we demonstrated that 255 DEGs were up/down-regulated in prostate tumors compared with BPH tissues; qRT-PCR also confirmed that the DEGs such as ITGBL1, KRT15, TGM4, and HOXA7 genes were up/down-regulated in PCa tissue and cell lines. The gene discussed is TGM4; the disease is benign prostatic hyperplasia.