Vicinity to the previously annotated genes allowed us to identify a diverse set of interactions between the 31 novel transcripts and the known genes relevant to melanoma, such as fusion transcripts (e.g. CUL5-ACAT1, NOX4-GRM5), novel exons (e.g. ACAT1, TYR, ARNT1, MCL1), bidirectional transcription from the same promoter (e.g. ENSA), and antisense lncRNAs (e.g. ADAMTSL4) (Fig. 4a, Additional file 1: Table S6). The gene discussed is NOX4; the disease is melanoma.