In addition, Patel et al. also found that loss of expression of genes that have key roles in IFN-γ signaling, as well as in antigen presentation, serve as a main mechanisms of tumor immune evasion and are underlying causes for cancer resistance/non-responsiveness to immunotherapies by using “two cell type” (2CT)-CRISPR assay [78]. The gene discussed is IFNG; the disease is cancer.