Additional confirmation of these results was shown in HeLa cervical cancer cells and in osteosarcoma U2OS cells that expressed the highest mRNA levels for WNT10B expression than in the control WNT10B low and/or negative cell lines, including MCF-7 (ER+), MCF-10A (TNBC control “normal”) or MG63 (osteosarcoma). The gene discussed is ESR1; the disease is osteosarcoma.