ESR1 and osteosarcoma: In contrast, MG-63 cells (another osteosarcoma cell line) had no detectable expression of WNT10B mRNA, similar to either MCF-7 (ER+) and MCF-10A (a TNBC control “normal” cell line), and MG-63 cells responded robustly to JA exposure as compared to U2OS cells (S1D Fig).