The results showed that with an increasing flaccidoxide-13-acetate concentration, the levels of FAK, phosphorylated-PI3K, phosphorylated-AKT and phosphorylated-mTOR decreased in T24 and RT4 cells (Figure 4), suggesting that the inhibition effect of flaccidoxide-13-acetate on T24 and RT4 bladder cancer cells is associated with reduction of the activities of FAK/PI3K/AKT/mTOR signaling proteins. The gene discussed is AKT1; the disease is urinary bladder carcinoma.