To study the effects of AML growth on BM vasculature and HSCs as disease propagates through the tissue, we used the well-established MLL-AF9-driven murine model of AML, which recapitulates phenotypic and pathological features of human MLL-rearranged AML (Krivtsov et al., 2006, Somervaille and Cleary, 2006). Here, KMT2A is linked to acute myeloid leukemia.