Two months after the first B16K1 melanoma cell inoculation, B16K1 melanoma cell re-injection in the surviving TNF- and TNFR1-deficient mice did not compromise overall survival, demonstrating that animals were totally vaccinated towards B16K1 melanoma cells (Fig. 1c and Supplementary Fig. 1c). Here, TNFRSF1A is linked to melanoma.