Correspondingly, the expressions of p62, beclin-1, LC3II/LC3I ratio, P-AMPK and P-ULK1 were all reversely altered in line with the changes of these proteins as observed in vitro in HCT116 cells, pinpointing an AMPK/ULK1-mediated autophagy inhibition in tumor tissues of the mice with the combination treatment (Fig. 6b). Here, SQSTM1 is linked to neoplasm.