When endogenous EphA2 was inhibited by miR-302b in the cells co-expressing miR-302b and EphA2, the number of cancer cells migrating through the Matrigel decreased significantly compared with the EphA2 overexpression group, while miR-302b over-expression group showed the opposite effect comparing with the control group (P < 0.05). The gene discussed is EPHA2; the disease is cancer.