POLA2 and melanoma: These included essential components of the pre-replication complex (e.g., CDC6, MCM4/6), as well as critical factors for initiation and progression of DNA replication (including DNA2, POLA1, POLA2, RPA2/3, RFC4/5, CDC20, and GINS2/4), precisely the phenotype observed for CELF1 depletion in melanoma cells (Fig. 2c, d).