NRAS and metastatic melanoma: RNA sequencing (RNA-Seq) was then set to assess gene expression differences in normal skin melanocytes and the well-characterized UACC-62, SK-Mel-147, and SK-Mel-28 cell lines, representative of metastatic melanomas with prototypical mutations in BRAF, NRAS, and p53 respectively21 (Supplementary Table 1).