We applied the higher‐throughput TileSeq approach, coupled with yeast complementation, to a diverse set of genes: SUMO1, for which heterozygous null variants are associated with cleft palate (Andreou et al, 2007); thiamine pyrophosphokinase 1 (TPK1), associated with vitamin B1 metabolism dysfunction (Mayr et al, 2011); and CALM1, CALM2, and CALM3, associated with cardiac arrhythmias (long‐QT syndrome (Crotti et al, 2013) and catecholaminergic polymorphic ventricular tachycardia (Nyegaard et al, 2012)). Here, CALM2 is linked to chronic obstructive pulmonary disease.