We applied the higher‐throughput TileSeq approach, coupled with yeast complementation, to a diverse set of genes: SUMO1, for which heterozygous null variants are associated with cleft palate (Andreou et al, 2007); thiamine pyrophosphokinase 1 (TPK1), associated with vitamin B1 metabolism dysfunction (Mayr et al, 2011); and CALM1, CALM2, and CALM3, associated with cardiac arrhythmias (long‐QT syndrome (Crotti et al, 2013) and catecholaminergic polymorphic ventricular tachycardia (Nyegaard et al, 2012)). The gene discussed is CALM3; the disease is chronic obstructive pulmonary disease.