Recently, PTEN was found to be direct target of miR-221 and miR-222, which induce TRAIL resistance and enhance HCC cell migration.57 miR-221 and miR-222 also regulate the expression protein phosphatase 2A subunit B and TIMP3 tumor suppressors, thus activating the AKT pathway and metallopeptidase to promote HCC cell invasion and meta-stasis.58 Some investigators reported that miR-30d and miR-151 involved in HCC invasion and metastasis.59, 60 The miR-30d is frequently upregulated in HCC and its expression is associated with intrahepatic metastasis.60 This evidence concerns the gene TIMP3 and hepatocellular carcinoma.