To further characterize the critical role of SHIP-1 and Lyn during bacterial infection, we cross-bred Ship-1−/− mice with Lyn−/− mice to generate a double gene knockout mouse (Lyn−/−/Ship-1−/−, short as L/S−/−, Supplementary Figure 1A), and then challenged with PAO1 (10 mice per group). Here, LYN is linked to bacterial infectious disease.