LYN and infection: In addition, 2 h post PAO1 infection, 3-(4,5-dimethylthiazol-2-yl)-2,5-dimethyltetrazolium bromide assay showed a significant decrease in the survivals of Lyn- and Ezrin-silenced MH-S cells, respectively (Figure 2j), indicating that the IL-6R–Lyn–Ezrin complex axis negatively regulates P. aeruginosa-induced host inflammatory responses.