In a study of human colorectal carcinoma specimens detailing the relationship between T-cell densities at the invasive tumor margin and those in the center of the tumor, high densities of CD45R+CD3+CD8+granzyme+ T cells (antigen-specific cytolytic Teff cells) were associated with a lower possibility of tumor relapse and improved overall survival.47 To further investigate the antitumor mechanism, we analyzed DC, MDSC, MΦ, Teff/Treg changes in the spleen, lymph node and tumor samples by FACS. Here, CD8A is linked to colorectal carcinoma.