In order to assess the prepared tumor cell vaccines are in accordance with the optimized condition ‘no tumorigenicity but secreting cytokines’, we collected the cell supernatants after 48 h from cell vaccine irradiated with a sublethal dose X-ray and detected expression of PD-1 antibody and GM-CSF by ELISA, the schedule for vaccine preparation as shown in (Figure 2a). This evidence concerns the gene CSF2 and neoplasm.