In order to further confirm the functional role of TIFA in HCC, we examined apoptosis in the HCC tumor cell line SK-Hep-1 following TIFA reconstitution by lentivirus for 48 h and after several passages post infection (>7 days) using (PI-Annexin V double staining) flow cytometry. The gene discussed is ANXA5; the disease is hepatocellular carcinoma.