All vaccinated mice were able to control parasitemia, remarkably the highest reductio﻿n (6-fold) was observed in Traspain vaccinated mice (mean area under the parasitemia curve ±SEM, c-di-AMP: 330±20, Traspain+c-di-AMP: 54±9, Nt-Cz+ASP2+c-di-AMP: 102±22, ASP2+c-di-AMP: 95±24, Nt-Cz+c-di-AMP: 91±12) (Fig. 6a). This evidence concerns the gene BACE1 and parasitic infectious disease.