PIK3CA and cancer: When applied on 40 ng of DNA from fresh HCT116 cells (Fig. 3a), eight variants were detected, including KRAS (p.G13D), PIK3CA (p.H1047R), and CTNNB1 (p.S45del), with all of them being reported in ATCC or in COSMIC (Catalog of Somatic Mutations In Cancer).