MAPT and tauopathy: To answer these questions, we used several in vitro and in vivo disease models, (1) mouse primary cortical neuronal cells treated with rotenone, (2) tauopathy model HEK293 cells overexpressing tau residues 244 to 372, with mutations of P301L and V337M exposed to recombinant tau fibrils that indefinitely propagate tau aggregates (aggregate-positive cells, AP).