CD86 and neoplasm: In the CD11b+ enriched population, there were significantly more CD11b+ Ly6G-GR1-F480intCD11c- macrophages in tumours compared to normal mammary glands (Fig. 2C), which may be recruited macrophages and more of these CD11c-F480int macrophages exhibit high expression of CD206 and low expression of CD86 (Fig. 2A,D), suggesting that TAMs in the MMTV-Wnt tumours may be more M2 polarized.