CIS cells as PGCs and gonocytes, express transcription factors associated with embryonic stem cell pluripotency, such as POU5F1/OCT-3/4, NANOG, T1A-2, MYCL1, GDF3, LIN28-A, DPPA4, DPPA5, KIT and AP-2g. This evidence concerns the gene POU5F1 and in situ carcinoma.