Moreover, four KEGG pathways were over-represented in protein digestion and absorption, ECM-receptor interaction, amoebiasis, focal adhesion and PI3K-Akt signaling pathway, and down-regulated DEGs enriched in sulfur metabolism, metabolic pathways, fatty acid degradation, starch and sucrose metabolism and drug metabolism - cytochrome P450 (Table 2). The gene discussed is AKT1; the disease is amebiasis.