We designed two single-guide RNAs (sgRNAs), sgRNA-1 and -2, to target exon 2 and exon 3 of human WAVE3, respectively (Figure 1A) in human MDA-MB-231 BC cells, a widely used model for TNBC that has elevated levels of WAVE3 [14, 35]. The gene discussed is WASF3; the disease is breast cancer.