We also investigated the effect of SR144528, a selective antagonist of CB2R, on the development of pulmonary fibrosis in vitro and vivo. Although SR144528 could reverse TGF-β1+JWH133 group’s trend in TGF-β1 induced mice lung fibroblasts proliferation and migration, SR144528 could not reverse the trend in α-SMA protein expression level in vitro. This evidence concerns the gene TGFB1 and pulmonary fibrosis.