Expression of TBC1D9 and CEACAM6 was higher in GATA3 mutant MCF-7 cells, GATA3 mutant CAMA1 cells, and primary GATA3 mutant breast cancer cells, whereas expression of PPT1, CYBRD1, HOXC13, and AFF3 was higher in GATA3 wild type cells (Figures 4 and 5). This evidence concerns the gene AFF3 and breast cancer.