EGFR and familial pancreatic carcinoma: To provide further evidence that decreased EGFR levels are required for CuB-induced growth inhibition in pancreatic cancer cells, we performed lentiviral shRNA knockdown of EGFR in BxPC-3 and HPAC cells (EGFR-shRNA), which was compared to non-target control cells (NTC-shRNA) by Western blotting analysis (Figure 3E).