On the other hand, the PI3 Kinase inhibitor wortmannin suppressed SOX2 expression in EBC2 lung SCC cells and in TE1, TE10 esophageal SCC cells whereas ATF/SOX2 did not alter phosphorylated AKT (pAKT) expression, suggesting that pAKT might be an upstream regulator of SOX2 in these kinds of cells (Supplementary Figure 7) [33, 34]. This evidence concerns the gene SOX2 and esophageal squamous cell carcinoma.