In three cases, the pyrosequencing method failed to detect the EGFR mutations on TMA biopsy cores (% tumor cells, range 10 to 40%), whereas the analysis on the corresponding whole tissue sections having more than 50% of tumor cells demonstrated the same mutations by the Idylla system (Cases 4, 25 and 37; Supplementary Table 1). Here, EGFR is linked to neoplasm.