Therefore, we introduced expression constructs encoding two LINC01021 transcript variants (isoforms #2 and #4, respectively, see Figure 2A) into the p53-proficient RKO and SW48 CRC cell lines, as well as their isogenic p53 knockout derivatives, that, in contrast to HCT116 cells, are suitable host cell lines for the episomal pRTR vector [37] and allow tight regulation of LINC01021 expression by doxycycline. This evidence concerns the gene TP53 and colorectal carcinoma.