Interestingly, Wnt/β-catenin genes, reported as PRMT5 targets in leukemic stem cells (Jin et al., 2016, Tee et al., 2010), could not be validated by qPCR (Wnt5A, PRICKLE2), implying that the mechanisms by which PRMT5 regulates stem cells in breast cancer and leukemia are different. The gene discussed is PRMT5; the disease is leukemia.