When the ratio of concentrations of cdh23 to pcdh15 was varied from 1:1 to 5:1, the ΔTm for the cdh23(WT)-pcdh15(WT) complex ranged from 1.8 ± 0.8°C to 5.5 ± 0.8°C, whereas for the complex involving the deafness mutant pcdh15(R113G), the ΔTm ranged from -0.6 ± 0.6°C to 1.5 ± 0.8 °C (Table 1 and Figs 3B, 3C and 4A). The gene discussed is CDH23; the disease is deafness.