As previously reported,16,36 there was no significant increase in RV hypertrophy (RVH) in HFD-fed ApoE−/− mice, and there were no significant differences in macitentan-treated HFD-fed ApoE−/− mice when compared with treatment-naïve ApoE−/− mice (data not shown). This evidence concerns the gene APOE and cardiac hypertrophy.