GFAP and Alzheimer disease: We also performed double-immunofluorescence microscopy for Aβ and GFAP or Aβ and IBA1 using frontal and temporal cortices prepared from 7- and 12-month-old, male and female 3xTg-AD-H mice, in comparison with non-Tg mice (Supplementary Figs S8 and S9).