We note that several genes involved in neuronal function, such as Egr3, Egr4, Fosl2, Grik1, Homer1, Lig4, Npas4, Nptx1, Pcsk1, Vegfa and Xbp1 were downregulated, especially in the 3xTg-AD-H cortex (Supplementary Tables S6 and S7), which may correlate with the previously reported cognitive impairment17,18. The gene discussed is LIG4; the disease is Alzheimer disease.