Furthermore, we provide evidence of a mechanism for cross-activation between the Ras/Raf/MEK/ERK and PI3K/AKT/mTOR pathways via Ras-suppressing PIK3IP1, a suppressor of the PI3K/Akt/mTOR pathway, in Ras-pathway activated cancer cells as well as in clinical samples from patients with colorectal and lung adenocarcinomas. This evidence concerns the gene PIK3IP1 and cancer.