Considering that TGF-β1/SMAD3 signaling pathway is a key mediator of HSC activation that leads to liver fibrosis [28], we further used a co-culture system of ADSCs together with HSCs to investigate whether ADSCs could suppress TGF-β1/SMAD3 signaling in HSCs. This evidence concerns the gene TGFB1 and Hepatic fibrosis.