TNF and melanoma: Furthermore, in melanoma patients, PD-1+ T cell clones are antigen-specific T cell clonotypes with higher functional avidity and reactivity (IFN-γ and TNF-α production after activation) than PD-1− T cell clones (37), and PD-1 expression can be used as a biomarker for neoantigen-specific T cells in TILs and in the peripheral blood (38–40).