Quantitative PCR was used to determine NGF, BDNF and receptors (TrkA, TrkB, p75NTR) and PACAP/VIP and receptors (PAC1, VPAC1, VPAC2) transcripts expression in LUT tissues from NGF-OE and WT mice with CYP-induced cystitis (4 h, 48 h, and chronic) (Girard et al., 2012). The gene discussed is ADCYAP1R1; the disease is cystitis.