After CYP-induced cystitis, additional changes in PACAP-EGFP+ nerves fibers and cells were observed in numerous supraspinal locations including: locus coeruleus, Barrington’s nucleus, rostral ventrolateral medulla, PAG, raphe, and amygdala (May et al., 2015, 2017a). Here, ADCYAP1 is linked to cystitis.