RAD51 and melanoma: To investigate the basis for the reduced RAD51 levels after cisplatin treatment of melanoma cells, cultures of A375, C32, G361, PEO4 and MRC5v1 cells were treated with 3 μM cisplatin for 24, 48 and 72 h before RNA was extracted and RAD51 mRNA levels were determined by quantitative RT-PCR.