Although significant pattern was not detected in the cancer antigen 19–9 (CA19–9), carcino-embryionic antigen (CEA) and AFP levels among GC patients with both negative and positive cytology serum and peritoneal lavage fluid [9], it was found that anemia and elevated levels of serum CEA, CA19-9, CA125 were frequently detected among 80% (28/35), 60% (21/35), 42.9% (15/35), 20% (7/35) of AFP-producing GC patients, particularly for those with serosal involvement, lymphatic and venous invasion, and lymph node metastasis [10]. This evidence concerns the gene AFP and metastatic malignant neoplasm in the lymph nodes.