Immunoblot analyses of pRictor, Rictor, pAMPK, AMPK, pRaptor, Raptor, pmTOR, mTOR, pAKT, AKT, pPTEN, PTEN, pp70S6K, p70S6K, pRPS6, RPS6 and p4E-BP1, 4E-BP1 revealed a significantly higher expression of these proteins in CRC compared to the corresponding NNT (Figure 8A and Supplementary Figure 28). The gene discussed is PTEN; the disease is colorectal carcinoma.