Moreover, the percentage of GPC1+ plasma exosomes in total plasma exosomes was significantly higher in CRC patients with relapse than patients without relapse, in CRC patients that died with relapse than in patients that survived with relapse, in CRC patients that survived from relapse than in patients that survived without relapse, and the GPC1+ plasma exosome counts progressively increased in relapsed colon cancer patients nine months after surgery. This evidence concerns the gene GPC1 and malignant colon neoplasm.