To determine the chronic effects of exogenously overexpressed KHSRP on ESCC cell function in vitro, we established stable transfectants that expressed HA-tagged KHSRP protein using KYSE1190 and KYSE1250 cells with relatively low expression of endogenous KHSRP (Figure 3A and 3B). This evidence concerns the gene KHSRP and esophageal squamous cell carcinoma.