In two ESCC tumors with positive cytoplasmic KHSRP immunoreactivity, miR-21, miR-130b, and miR-301a expression levels in tumors were higher and BMP6, PDCD4, and TIMP3 mRNA expression levels were lower compared with those in paired non-tumorous tissues, whereas these alterations were not consistently observed in two ESCC tumors with negative cytoplasmic KHSRP immunoreactivity (Supplementary Figure 6A, 6B). This evidence concerns the gene TIMP3 and esophageal squamous cell carcinoma.