Therefore we expressed RV’s cellular receptor JAM-A onto the BV envelope, allowed RV to bind to the BVJAM viruses, and tested the resulting BVJAM-RV complex for reovirus infection of JAM-A-deficient U-118MG glioma cells in two-dimensional standard cell-culture conditions and on three-dimensional tumour-cell spheroid cultures. Here, F11R is linked to central nervous system cancer.