In our study, the treatment of MOK pharmacopuncture in LT4-induced hyperthyroidism rats significantly decreased the body temperature, prevented the weight loss of liver, heart, and spleen, regulated the levels of thyroid hormones TSH, T4, and T3, and also decreased the serum levels of GOT, and GPT similarly to the PTU-treated group. This evidence concerns the gene TG and hyperthyroidism.