After treatment with Sirtinol (10 nmol, i.c.v.), the amount of TUNEL-positive neurons (Figure 9A) and the level of Bax (Figure 9B) in the hippocampus of cotreatment with NaHS and CRS-exposed rats were significantly increased, while the level of Bcl2 in the hippocampus of cotreatment with NaHS and CRS-exposed rats was significantly decreased (Figure 9C), indicated that Sirtinol abrogates the protective action of H2S on CRS-induced apoptosis. This evidence concerns the gene BAX and congenital rubella syndrome.