Our results indicated that calcitriol up-regulates the expression of JMJD3 in kidney cancer cell, and then JMJD3 further increases transcription of p16INK4A. It has shown that vitamin D metabolism is closely related to epigenetic modification such as histone acetylation [31], and the study further expands the view that vitamin D (calcitriol) also regulated histone methylation. This evidence concerns the gene CDKN2A and kidney cancer.