Interestingly, a number of hit genes from the genomics screen were also found to be upregulated in CCD-18Co fibroblasts only in the 3D co-culture tumor spheroid (e.g. ARAF, PKD2, OGR1, WNT5A; Figure 4A bold genes, Supplementary Figure 3 and Supplementary Table 2) and these lead targets were advanced into further discovery efforts (Supplementary Figure 2). This evidence concerns the gene GPR68 and neoplasm.