Several target genes, including ARAF and WNT5A, demonstrated modest phenotypes when knocked down in the monoculture tumor spheroids (Figure 6A, green bars), but most shRNAs produced no discernable phenotype when depleted in the cancer cells (Supplementary Figure 4A) in contrast to their profound effects when depleted in the fibroblast component (Figure 6A, red bars and Supplementary Figure 4A). This evidence concerns the gene ARAF and cancer.