From in vitro assays, we found that GPC1 committed to cellular proliferation or migration, that EVI1, KRAS and/or miR-96 regulated expression of GPC1, and that EVI1 modulated the oncogenic role of GPC1 in both pancreatic noncancerous cell lines and pancreatic cancer cell lines. This evidence concerns the gene KRAS and familial pancreatic carcinoma.