We examined the expression of GPC1 using real-time quantitative reverse transcription PCR (qRT-PCR) in 11 pancreatic cancer cell lines (BxPC-3, Capan-1, DANG, KLM-1, MIA PaCa-2, PANC-1, PK-1, PK-45H, PK-45P, PK-8, PK-9 cell lines) and HPDE cell lines (Figure 3A). This evidence concerns the gene GPC1 and pancreatic neoplasm.