To further validate the relationship between AQP1 and β-catenin, combinatorial analysis of AQP1 and β-catenin was evaluated by the same cohort of 160 astrocytoma specimens and we demonstrated for the first time that AQP1 expression positively correlated with β-catenin (rs = 0.158, P = 0.045, Table 5, Figure 5A–5B). The gene discussed is AQP1; the disease is astrocytoma (excluding glioblastoma).