PNPLA3 and metabolic dysfunction-associated steatotic liver disease: Most biomarker studies performed to date to evaluate NAFLD histology were not performed on fatty acids.[19–26] Several studies used lipidomics or metabolomics approaches, including a recent report proposing a panel of 3 metabolites and 2 lipids combined to PNPLA3 genetic variant for the detection of NASH [AUROC = 0.86].[26] However, some studies have measured fatty acids in plasma or in liver in the context of NAFLD.