To assess whether early inhibition of NF-κB-p65 nuclear translocation by ORFV119 involves de novo viral protein synthesis in the infected cells, OFTu cells were pre-treated with the protein synthesis inhibitor cycloheximide (CHX) for 30 min followed by infection with OV-IA82 or OV-IA82-Δ119 for 30 min and 1 h in presence of the drug. The gene discussed is NFKB1; the disease is infection.