To confirm the interaction in the context of the virus infection, OFTu cells were mock-infected or infected with OV-IA82-RV119Flag or OV-IA82-RV119LxGxE-Flag (MOI, 10), and cell lysates prepared at 12 h p.i. Reciprocal co-immunoprecipitation with either anti-Flag or anti pRb antibodies showed that ORFV119 but not ORFV119LxGxE-Flag co-immunoprecipitates with pRb (Fig 3C–3F). Here, RB1 is linked to viral infectious disease.